Cellular and Molecular Bioengineering

(E-196) Morphological response of activated microglia upon treatment with mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) is indicative of their functional changes

(E-198) Mapping Protein-Protein Interactions at the Blood-Brain Barrier with TurboID-Mediated Proximity Labeling

(E-199) Introducing Metabolic Pathways to Gut Bacteria to Engineer the Processing of Dietary Metabolites

(E-200) Mesenchymal Stromal Cells as a Potential Cell-Therapeutic Option for Endothelial Dysfunction Caused by Exposure to Ionizing Radiation

(F-201) Generation and evaluation of proteases with high substrate sequence specificity

(F-203) Optimization of CRISPR Cas 9 Delivery Using Electroporation

(F-204) Engineering of Genetically Encoded Calcium Channel Blockers

(F-205) Evaluating Mesenchymal Stromal Cell Mediated Extracellular Matrix Remodeling and Paracrine Signaling in Gelatin Maleimide Microgels

(F-206) Evaluation of precision and efficacy of CRISPR-mediated transgene integration at muscle-specific safe-harbor loci.

(F-207) Metabolic stress in human cell model of the aging associated disease glaucoma

(F-209) Microbioreactors for Facile Extracellular Vesicle Harvesting and Quantification of Vesicular Heterogeneity in Breast Cancer Tumor at Single-EV Resolution

(F-211) The Role of Substrate Stiffness on Diabetic Wound Healing

(F-212) Revolutionizing Estrogen Receptor Dimerization Monitoring with a Novel Fluorescence Resonance Energy Transfer-Based Biosensor

(F-213) Yes-associated protein partially mediates the anti-inflammatory transcriptome of endothelial cells induced by topographical micropatterning

(F-214) Revealing Spatial Subcellular Signaling Networks Using Multiplexed Protein Profiling

(F-215) Seven-Fold Increase Observed in AAV Transduction of Hepatocytes In Vitro due to Electric Pulse Delivery

(F-216) Stretch activation of yes-associated protein (YAP) in adipocytes

(F-217) Yes-associated protein (YAP) and piezo1 translocalization in fluid sheared MSCs

(F-218) Simultaneously measuring electrophoretic mobility and size of single particles using a novel microfluidic device based on AC electrophoresis

(F-219) Traction forces of upstream migrating CD4+ T-cell

(F-222) Spatially Resolved Cellular Senescence Mapping in Healthy and NASH Human Liver

(D-152) CD200 treatment prevents neuroinflammation in murine microglia

(D-153) Effects of Pulsed Electrochemical Fields on Retinal Progenitor Migration

(D-154) Engineering Novel Dual Decoy Receptor Antagonists for Neovascular Eye Diseases

(D-157) Computational Design of Specific Protein Binders with Varying Affinities for Interleukin-4

(E-188) Metabolically activated CAR-Macrophages based therapy as a treatment for lymphoma

(D-155) TRPV1-based Sonogenetics for Immunotherapy

(D-156) Engineering controllable universal chimeric antigen receptor T cells for solid tumor treatment

(D-150) Profiling Extracellular Matrix Degradation by Enterotoxigenic Bacteroides Fragilis Strains

(D-158) Adipocyte Extracellular Vesicle Uptake via Leukocytes and their Impact on the Immune Response

(D-151) Discover Essential Host SUMOyaltion Pathway for Virus Life Cycle Through qFRET/MS-based Technology

(D-159) Characterization of macrophage polarization states following shear stress exposure

(D-160) Biofloating: a cell-cell interaction platform for antibody discovery against multi-pass transmembrane proteins

(E-161) Divergent Role of Caveolin-1 in the Mineralization Mechanisms of Bone and Vasculature

(E-163) Characterization of mechanically tuned liposomal nanocarriers for cell and phenotype-specific endocytosis using Atomic Force Microscopy

(E-162) Donor-matched Multiorgan Microphysiological Systems for the Studies of Immunometabolic Diseases

(E-166) Elevated Rho Signaling Downstream of the R206H ACVR1 Mutation in FOP Alters Chromatin Organization

(E-164) Diversity in Contact Guidance Sensing by Cells of Different Motility Modes

(E-168) Effects of Extracellular Vesicles Derived from Non-Irradiated or Irradiated Skeletal Muscle Stem Cells on Myogenesis and Angiogenesis

(E-169) Differential roles of extracellular matrix proteins on primary and metastatic pancreatic cancer cells

(E-173) Determination of Microbial Guilds and Niches Enable Targeted Modifications of the Skin Microbiome

(E-170) Direction-dependent effects of nanomagnetic forces on the distribution of calcium hotspots in cortical neurons

(E-171) A novel DNA display technology that facilitates the engineering of nanobodies

(E-174) Differential regulation of Piezo channels in articular chondrocytes and nerve post ACL-injury

(E-175) Enhanced presentation of notch1 and α4β1 integrin ligands by interfacial polymeric coatings improves the emergence of T cell progenitors from human hematopoietic and pluripotent stem cells

(E-176) Left-right positioning of organelles influences chirality in micropatterned myoblast cells

(E-172) Design of Direct 26S Proteasome Recruiters with Language Model-Derived Guide Peptides

(D-137) EFFECTS of LOW ENERGY X-RAY RADIATION at VARYING DOSE RATES on HUMAN AORTIC SMOOTH MUSCLE in vitro

(E-167) Characterization of initial immune response by oral macrophages and their role in host-pathogen interactions

(D-138) A Microfluidic Device to Compare Shear Flow vs Chemokine Signaling Effects on Immune Cell Migration

(D-139) Evidence of a fourth actin-binding site on leiomodin-2

(D-132) Bioinformatics-guided design of transcriptional regulators for implementing decision-making behaviors in cellular systems

(D-134) Engineering gene circuits in immune cells as transplant rejection diagnostics with enhanced specificity and sensitivity

(D-133) Development of a lipid-conjugated substrate to measure the activity of protein kinase B in single cells by capillary electrophoresis

(D-135) Confined Migration Drives Stem Cell Epigenetics and Differentiation

(F-224) Physiological confinement induces the formation of mechanically-induced biomolecular condensates

(F-210) Repurposing Poloxamer P188 for TBI Treatment

(F-223) Reparative Role of Copolymer P188 in an Injured Brain Endothelium Model

(D-136) A novel DNA double-strand breaks biosensor based on fluorescence resonance energy transfer

(D-140) Anti-inflammatory (M2), but not pro-inflammatory (M1), macrophages can migrate against the direction of shear flow on the vascular endothelium

(D-141) Engineering Antibody–Invertase Fusion Proteins for Enhanced Detection of Diseases Targeted Antibodies Using Commercial Glucometers

(D-142) DNA-inspired Janus Base Nanopieces for In Vivo Gene Editing in Liver

(F-220) Stimuli-responsive Magnetic-plasmonic core-shell nanosensors for trimodal US/PA/MR spatial and functional tracking of cell therapy

(D-143) AAV-mDLX5/6 Vectors Transduce Cortical Neurons Expressing Parvalbumin-Expressing but not Calretinin-Expressing Interneurons

(D-144) Development of responsive element luciferase probes to measure real-time macrophage polarization

(D-145) BCG infection modulates the macrophage phosphoproteome to generate trained immunity

(D-146) Defining functional subtypes of cellular senescence at single-cell resolution

(D-147) Characterization of Effects of inkjet Bioprinting of Human Fibroblasts into Stem Cell Environment

(D-148) Engineering a novel IL-2-based immunocytokine with enhanced effector and memory function.

(D-149) Chromatin adaptation to confinement during microchannel-based cell migration

(F-231) Simultaneous Genomic Locus Imaging and DNA Repair Monitoring by Elastin-like Proteins in Live Cells

(F-232) Role of The Mechanosensitive PIEZO1 Channel in Microglia Activation

(F-233) Regulation of Wound Closure via Feedback between Fingering Patterns and Dynamic Behaviors of Cells

(F-234) Single-cell Detection of Proteins and Their Spatial Distribution on Mice Tissue using a Novel Microchip Assay

(F-225) SPNS1 mediates lysophosphatidylcholine transport that enables cell survival under choline limitation

(F-226) Visualizing and Manipulating a Stochastic Epigenetic Switch Controlling T cell Lineage Commitment at Single-locus Resolution

(F-227) T Cell Mechanosensing and Costimulation Programs Harnessed for Optimal Ex Vivo Expansion

(F-228) Utilization of EPM2A as a Target for Gene Replacement Therapy in Lafora Disease

(F-230) Therapeutic potential of nanoceria pretreatment in preventing the urological chronic pelvic pain syndrome: Immunomodulation via reactive oxygen species scavenging and SerpinB2 downregulation.

(F-221) Studying Effects of Disease-related Mutations in Progranulin Using Molecular Dynamics Simulations and Circular Dichroism

(F-229) Transplantation of muscle stem cell mitochondria augments the bioenergetic function of dystrophic muscle in mice

(H-288) Tumors lacking the Macrophage Immune checkpoint ligand CD47 grow as normal but can be eliminated by Antibody-opsonization

(H-289) A Glycogen Synthase Kinase-3 Inhibitor Attenuates cGAMP Induced CXCL10 Production by Peripheral Blood Mononuclear Cells

(H-290) Investigating regulatory cross-talk and interference in large, multi-gene synthetic loci in mammalian cells

(H-291) Optimization of CRISPR/Cas-based programmable DNA methylation in human primary T cells for regulation of immunological exhaustion

(H-292) An in vitro alveolar-capillary model to investigate the therapeutic potential of cdc42 binding kinase MRCKα for treatment of Acute Respiratory Distress Syndrome

(H-293) Engineer ultrasound-controllable CAR-T cells with enhanced sustainability and inducibility for solid tumor therapy

(H-294) The effect of lung cancer-associated fibroblasts on the functionality of vasculature

(H-295) Engineering Focused-Ultrasound Controllable Protease-Activable Tumor Cells

(H-296) Heat-Inducible CAR-T Overcomes Adverse Mechanical Tumor Microenvironment in 3DBioprinted Glioblastoma Model.

(H-297) Chromatin Conformation Mediates Plasticity and Impacts Post-Chemotherapy Cancer Cell Fates.

(H-298) Doxycycline as Prophylactic Treatment for Prevention of Severe COVID-19 Induced Symptoms and Other Inflammatory Diseases

(H-299) Deciphering Force-induced Protein Interactions Surrounding Keratin Fibers Using Microneedle Stretch

(H-300) Microbial teamwork: circuits for in situ differentiation from seed cells

(H-301) High-Throughput Characterization of Heterologously-Expressed Membrane Transporter Proteins: A Snorkel Tag Approach

(H-302) Control of CAR-T Therapy of Solid Tumors under Hypoxia via Focused Ultrasound

(H-303) Compression sequencing for deep and scalable single-cell multi-omics

(H-304) Non-invasive imaging of gut microbes with reversibly switchable photoacoustic protein

(H-305) Unraveling the Dynamic Interplay: ECM, Cancer Cell Invasion, and Angiogenesis in the Tumor Microenvironment

(H-306) Slowly, Then All at Once: Cellular Dynamics and Potential Limits of Transient Reprogramming in Human Fibroblasts

(H-307) Engineering antibodies with light-sensitive domains towards in situ regulation of binding affinity

(H-307) Peptoids as Potential Therapeutics Against RAGE-Associated Neuroinflammation in Alzheimer’s Disease

(H-308) Optimization and Validation of Utilizing Alginate-Based Casts to Model and Characterize the Physical Properties associated with Bioprinted Tissue Constructs

(H-308) Optogenetic Tools for Studying Endothelial Cell Mechanobiology

(H-309) The Role of Intra-tumoral Compressive Stress on Glioblastoma Malignancy

(H-310) Evaluating the Single Cell Transcriptomic Profiles of an iPSC-derived Neuron-Astrocyte Assembloid Exposed to Tauopathy Vs. Synucleinopathy

(H-311) Assessing the Growth Dynamics of a Mock Community with Pseudomonas aeruginosa and Staphylococcus aureus

(H-312) Exploring the Link Between Traumatic Brain Injury and Alzheimer’s Disease

(H-313) Effects of low-energy x-rays and UV radiation on fibroblast cells

(H-314) Unraveling the Q system function in prokaryotes

(H-315) Development of a Sample Adequacy Control for a Human Papillomavirus 16 Rapid Test

(H-316) Validation of Bidirectional Promoters as a Novel Tool for Transgene Coexpression

(H-317) Ovarian Cancer Cells Display Altered Proliferation Expression when Exposed to Strain and Chemotherapy Drugs

(H-318) Optimization of the assembly for a disassemble milli-fluidic bioreactor

(H-319) Investigating the effects of fluid shear stress on the progression of post-partum breast cancer

(H-320) Holotomographic microscopy - a platform to investigate the role of the adhesome in endothelial cell metabolism

(I-321) Engineering Synthetic Phospho-Signaling Networks in Mammalian Cells

(I-322) Controlled Assembly of Synthetic Cells using Megamolecules

(I-323) Investigating the Penetration of Collagenase-Attached ICG Liposomes Through Gelatin Hydrogels Using Photoacoustic Imaging

(I-324) Developing RNA-based sensors for cell-specific detection and manipulation using ADAR to improve liver organoids

(I-325) Evaluation of engineered promoters for homology-independent targeted integration of curative promoter-driven transgenes for hemophilia therapeutics

(I-326) Effects of Matrix Stiffness-induced Hepatic Stellate Cell Activation on Adhesion Region Stiffening

(I-327) Effect of Receptor for Advanced Glycation End-Products Inflammatory Response in Alzheimer's Disease

(I-328) Force-dependent Proximal Biotinylation Surrounding Actin Filaments in Live Cells

(I-329) Using Patient-Derived Samples to Analyze the Correlation Between Peritoneal Stiffness and Ovarian Cancer Metastasis

(I-330) Regulation of Muscle Stem Cell Structure and Function by Piezo1

(I-331) Unraveling the Role of Formins in Podocyte Dynamics and Function

(I-332) The Effect of High Fat Diet on Glucose Tolerance in ATP Sensitive Potassium channels in Dominant Negative Mice

(I-333) 3D Culture Model to Determine Wound Closure Rates

(I-334) Rational engineering of multimeric constructs for ultra-sensitive point-of-care diagnostics against Ebola secreted glycoprotein

(I-335) Natural Killer Cell Derived Small Extracellular Vesicles for Glioblastoma Therapy

(I-336) "Impact of 3-D Confinement on the Migratory Behavior of Young and Aged Cells: Implications for Cellular Senescence and Age-Related Decline"

(I-337) Proline restores senescence-associated loss of mitochondrial function

(I-338) Optimizing perfusable and stable 3D microvascular network within human bone marrow-on-a-chip devices by using alternate endothelial cell sources

(I-339) Generation of Fat-Cartilage Microphysiological Model as a New Tool to Study Obesity-Associated Osteoarthritis

(I-340) Probiotics formulation and delivery using nanocultures

(I-341) Effects of THC on Endothelial Cell Barrier Function

(I-342) Lipid Comparison Between Lab-Made and Murine In Vivo Clots and Correlation with Immune Response

(I-343) MRTF-SRF interaction promotes breast cancer cell migration in a DIAPH3-dependent manner

(I-344) Exploring the DNA damage repair kinetics from different damage agents

(I-345) The Development of a Mechanical Stimulation System for the in Vitro Knee Joint Model

(I-346) Migration Based Sorting of Breast Cancer Cells Reveal Bi-Directional Relationship with Epithelial to Mesenchymal Phenotypes

(I-347) Investigating the Effects of Transient Reprogramming of Aged Bone Marrow Stromal Cells In Vitro

(I-348) Studying Temporal Changes in Autophagy and Zika Virus Replication using Image-Based Profiling

(I-349) Investigating Obesogenic Effects of Common Artificial Food Colors on Adipose Tissue

(I-350) Global Methylation Analysis of Inkjet Bioprinted Human Dermal Fibroblasts Reveals Increased Methylation at the 6-hour Time Point

(I-351) Exploring Tunable Substrates Combined with Expansion Microscopy

(I-352) Probing the effects of ECM stiffness on hypersialylation and drug response in breast cancer

(I-353) Validation of PGBD5 as a DNA Transposable Element Required for Nervous System Development

(I-354) Development of a Blood-Brain Barrier on a Chip Model for Drug Delivery Studies

(I-355) Computational model to predict the pressure effects of ultrasonic waves on cytoskeletal reorganization in plant cells

(I-356) Effects of Pancreas Cancer Cell K-Ras G12D Inhibition on Cancer-Associated Fibroblast Heterogeneity in Hypoxic Conditions 

(I-357) DNA-based micropatterning for epithelial-mesenchymal interface mechanobiology

(I-358) Exploring the Impact of Multipoint Mechanical Force on Bone Tissue Through a Novel Bioreactor

(I-359) Breast Tumor Cell Migration under the Influence of Electric Field and Osmotic Pressure

(I-360) PolyloxExpress barcoding and tracking to examine adult mouse bone marrow endothelial cell differentiation potential

(J-361) A novel optogenetic split-enzyme system to control protein-protein interactions in the secretory compartment of cells

(J-362) The Impact of Charge regulation and the Ionic Intranuclear Environment on Chromatin charge and Structure

(J-363) Engineering of the direct electron transfer (DET) type glucose dehydrogenase to improve DET ability in the absence of an electron transfer subunit

(J-364) Engineering a human endothelium platform for disease modeling

(J-365) In-Plane Tissue Stretcher with Force Characterization and Optical Capabilities

(J-366) The RNA-binding protein CUGBP Elav-like family member 4 (CELF4) is a negative regulator of nociceptor excitability and hyperalgesia in mice

(J-367) Cancer-specific SRC activity profiling using exogenous artificial peptide probes

(J-368) Advancing Hypoxia Resistance in Stem Cell-Derived Islets for Type 1 Diabetes Therapy