Digital Magnetic Proximity Extension RPA-CRISPR/Cas12a-Aided Immunoassay with Attomolar Sensitivity

Saturday, October 14, 2023

8:45 AM – 9:00 AM PDT

Location: 342 (Center, Level 3)

Presenting Author(s)

Fangchi Shao, BSE

Ph.D. Candidate
Johns Hopkins University
BALTIMORE, Maryland, United States

Co-Author(s)

Jiumei Hu (she/her/hers)

PhD Candidate
Johns Hopkins University
Baltimore city, Maryland, United States
KH

Kuangwen Hsieh

Assistant Research Professor
Johns Hopkins University, United States
PZ

Pengfei Zhang

PhD Candidate
Princeton university, United States
Patarajarin Akarapipad, MS (she/her/hers)

Graduate student
Johns Hopkins University
Baltimore, Maryland, United States
JP

Joon Soo Park

Johns hopkins university, United States

Primary Investigator(s)

JW

Jeff Tza-Huei Wang

Principal Investigator
Johns Hopkins University, United States

We developed a digital magnetic proximity extension RPA-CRISPR/Cas12a-aided immunoassay for protein detection, employing magnetic beads to minimize background noise, triple antibody binding for increased specificity, and RPA-CRISPR/Cas12a technology to enhance sensitivity. We successfully detected the IL-8 protein biomarker with a limit of detection of 309.28 aM.