Professor Penn State University University Park, Pennsylvania, United States
Introduction:: 3D Bioprinting is a disruptive technology enabling deposition and patterning of living cells in order to manufacture replacement tissues and organs for tissue engineering, regenerative medicine, disease modeling and drug screening purposes. Despite great advances, bioprinting of scalable vascularized constructs for fabrication of robust perfusable in-vitro models or scalable tissues remains a challenge.
Materials and Methods:: In this talk, Dr. Ozbolat will survey the emerging field of bioprinting and its impact on medical sciences and present a wide range of 3D bioprinting efforts in biomanufacturing of tissue/organ substitutes performed in his laboratory in the last 12 years. Next, he will discuss the bioprinting efforts for building vascularized tissue models, such as tumor models for CAR-T cell-based immunotherapy and demonstrate a novel 3D printing approach for generation of vascular networks in scalable tissue constructs. Then, he will present intraoperative bioprinting for repairing composite soft/hard craniomaxillofacial tissues in a surgical setting (Figure 1) [1] and demonstrate a novel micro-surgery-based vascularization tactic for rapid angiogenesis of intraoperatively bioprinted tissues.
Results, Conclusions, and Discussions:: The results demonstrated that vascularization enables the reconstruction of scalable tissues, which can facilitate the perfusion of CAR-Ts through a multi-scale vascular network [2] or improve the tissue survival and function when bioprinted intraoperatively. Overall, 3D bioprinting holds great promise in tissue engineering and regenerative medicine, and building novel vascularization tactics enabled or integrated with bioprinting will allow the fabrication of functional scalable tissue substitutes.
Acknowledgements (Optional): : This work has been supported by NSF Award 1914885, NIH Awards R01DE028614, U19AI142733, R01EB034566, R21CA224422 and 2236 CoCirculation2 of TUBITAK award # 121C359.
References (Optional): :
Moncal, K.K., Gudapati, H., Godzik, K.P., Heo, D. N., Kang, K., Rizk, R., Ravnic, D., Wee, H.B., Pepley, D.F., Ozbolat, O., Lewis, G., Moore, J.Z., Driskell, R., Samson, T., Ozbolat, I.T., 2021, “Intra-operative Bioprinting for Hard, Soft, and Hard/Soft Composite Tissues for Craniomaxillofacial Reconstruction” Advanced Functional Materials, 2010858.
Dey, M., Kim, M.H., Dogan, M., Nagamine, M., Kozhaya, L., Celik, N., Unutmaz, D., Ozbolat, I.T., 2022, "Chemotherapeutics and CAR-T cell-based immunotherapeutics screening on a 3D bioprinted vascularized breast tumor model" Advanced Functional Materials, 202203966.
