Professor Chung-Ang University Anseong-si, Kyonggi-do, Republic of Korea
Introduction:: The overproduction of hydrogen peroxide (H2O2) is closely associated with the progression of numerous diseases, and thus, it is an important diagnostic marker for in vivo molecular imaging. Although chemiluminescence (CL) imaging with L-012 as a CL agent has been employed to detect H2O2, the low reactivity of H2O2 with L-012 and the short CL emission time of L-012 limit the in vivo applications for H2O2 detection. Recently, several reports have suggested that slow-release and controlled CL systems are desirable for intensive and long-lasting CL emissions for sensitive and accurate applications. In this study, we designed a highly sensitive, strong, and long-lasting CL nanoparticle system, Co2+ (catalyst)-doped L-012 (CL agent)/magnesium peroxide (MgO2) (L-012/Co@MgO2), for H2O2 detection in vivo.
Materials and Methods:: A novel Co2+-doped L-012/magnesium peroxide-based CL nanosystem (L-012/Co@MgO2) is designed. The structure and morphology of the nanosystem (L-012/Co@MgO2) was confirmed by XRD, UV-Vis spectroscopy, FT-IR, SEM and TEM techniques. The CL performance of L-012/Co@MgO2 nanosystem was analyzed against various H2O2 concentrations using a small animal imaging system and a fluorescence spectrometer with the Xe lamp turned off. In addition, in vivo H2O2 imaging in lipopolysaccharides (LPS)-induced inflammatory disease model and in vitro screening of H2O2 scavenging activity of antioxidant was assessed using L-012/Co@MgO2 nanosystem by a small animal imaging system.
Results, Conclusions, and Discussions:: The prepared L-012/Co@MgO2 is decomposed in aqueous solution and releases L-012 and Co2+, and the highly reactive hydroxyl radicals produced by the Co2+-catalysed H2O2 decomposition react with L-012 to generate strong and long-lasting CL emissions. In addition to visually detecting and quantitative analyzing H2O2, the biocompatible L-012/Co@MgO2 CL system can successfully realize H2O2 detection in LPS-induced inflammatory disease model and in vitro screening of the H2O2 scavenging activity of antioxidants. Our CL system has potential applications for the biosensing and bioimaging of highly produced H2O2 in various diseases.
