(G-246) Studying Responses of Multiple GBM Cell Lines to Extracellular Components

Introduction:: Glioblastoma multiforme (GBM) is among one of the most deadly, aggressive, and invasive types of cancer. Currently, there is no cure for patients diagnosed with GBM. Due to the cancer’s high rates of motility and invasion, even with treatment, patients are often given a survival time of 12-15 months after diagnosis. This makes GBM of particular interest to scientists who intend to improve outcomes of GBM patients.


Materials and Methods:: In this study, we utilized the nonadherent method and microfluidic channels to generate a three-dimensional tumor model of GBM and the brain’s tumor microenvironment. In this model, the GBM spheroids mimicking the primary tumors were introduced to a PDMS device which has microfluidic channels and matrigel to simulate physical confinements and the extracellular matrix in the brain tissue, respectively.

Results, Conclusions, and Discussions:: Using the model, we were able to observe the migration and sprouting behavior of four different GBM cell lines, U-118 MG, U-87 MG, LN-229, and A-172. The success of this project will allow us to learn more about the invasion of GBM cancer so that better treatments can be developed to prevent the progression of GBM infiltration and recurrence.

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